Prior to magnetic separation, cells were prestained using nucleic acid DAPI dye and after cell trapping inside the microchambers, captured cells were washed two times with PBS (with the flow rate of 2 µL min −1) to ensure the elution of the nonmagnetic labeled cells. Followed by this step side magnets were removed and PBS back-flow washing ...
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Here, we present a novel technique to immobilize magnetic particles onto whole G. oxydans in situ via a synthetic adhesive biomimetic material inspired by the protein glues of marine mussels. Our approach involves simple coating of a cell adherent polydopamine film onto magnetic nanoparticles, followed by conjugation of the polydopamine-coated nanoparticles to G. …
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In-situ cell recovery by using immobilized cells instead of free floating fermentation has introduced as a valuable strategy for elimination of centrifugation and filtration units. …
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A simple and rapid harvesting method by in situ magnetic separation with naked Fe 3 O 4 nanoparticles has been developed for the microalgal recovery of Botryococcus braunii and Chlorella ellipsoidea.After adding the magnetic particles to the microalgal culture broth, the microalgal cells were adsorbed and then separated by an external magnetic field.
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In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe 3 O 4 nanoparticles (NPs). The Fe 3 O 4 NPs were synthesized by coprecipitation followed by modification with ammonium oleate.
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The functional magnetic nanotubes were easily separated from the racemic solution using an external magnetic field which demonstrated its feasibility of recycling the adsorbent. All processes including in situ immobilization, enantioseparation (enantioenrichment) and magnetic separation were done by single process in a short time (only 10 min).
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Wu et al. [66] proposed a simple and new procedure to synthesize magnetic Fe 3 O 4-chitosan nanoparticles by in-situ synthesis method and used as support material for the immobilization of lipase enzyme using glutaraldehyde as cross-linker. In this process, ferrous ions were added to the combination of chitosan and sodium tripolyphosphate solution.
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In situ magnetic capturing and surface-enhanced Raman scattering (SERS) detection techniques can be used for detecting microbial contamination and uremic toxins. Magnetic SERS substrates were fabricated using immobilized gold nanoparticles (AuNPs) and iron-oxide (Fe 3 O 4) nanoparticles on exfoliated nanoscale silicate platelets (NSPs).The …
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Abstract A novel core–shell-structured Ag-based magnetic functional polymer nanocatalyst, i.e., Fe3O4@SN/GLA@chitosan-Ag (SN represents animo-functionalized SiO2, GLA signifies glutaraldehyde), was fabricated via a simple and environmentally friendly method. The magnetic separation property of Fe3O4, the protection effects of SiO2, the reduction and …
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Raspberry-like colloidal polymer/magnetite/silica composite microspheres were rationally fabricated based on in situ growth or interfacial immobilization of magnetic nanoparticles (MNPs) onto the ...
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Magnetic chitosan/Fe 3 O 4 nanoparticles prepared by in situ mineralization of Fe 3 O 4 in chitosan hydrogel were first modified by 3-aminopropyltriethoxysilane (3-APTES) and then tested for the possibility of being used as carriers for the immobilization of pullulanase. It was found that the immobilized enzyme showed high activity retention (>70%) and the affinity …
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In-situ stabilization is commonly used as a gentle option for environmental risk control of As contaminated soils (Fang et al., 2016; ... Further study needs to be considered on this immobilization/magnetic separation technique, such as application to different types of soil, the screening or optimization of magnetic materials, and the ...
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In situ growth technology can realize the nucleation and growth of TiO 2 on the film, which improves the binding force between the catalyst and the matrix while effectively suppressing the agglomeration of TiO 2.It provides an approach for preparing high-performance polymer/TiO 2 hybrid photocatalytic films. However, anatase TiO 2 with high photocatalytic …
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These factors could make the magnetic separation less effective. ... In situ preparation of magnetic Fe 3 O 4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in ... Characterization and optimization of mesoporous magnetic nanoparticles for immobilization and enhanced performance of porcine pancreatic lipase. ...
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Zero-valent iron (ZVI) facilitated in-situ selenium (Se) immobilization and its recovery by magnetic separation: ... A novel approach that combines ZVI reduction and magnetic separation for in-situ Se remediation and recovery was demonstrated. With ZVI addition (group A), both Se(VI) removal processes and precipitation were promoted in the ...
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In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe (3)O (4) nanoparticles (NPs). The Fe (3)O (4) NPs were …
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Zero-valent iron (ZVI) facilitated in-situ selenium (Se) immobilization and its recovery by magnetic separation: Mechanisms and implications for microbial ecology. Journal of Hazardous Materials, 473, Article 134591.
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The functional magnetic nanotubes were easily separated from the racemic solution using an external magnetic field which demonstrated its feasibility of recycling the adsorbent. All processes including in situ immobilization, enantioseparation (enantioenrichment) and magnetic separation were done by single process in a short time (only 10min).
WhatsApp: +86 18221755073
The immobilization of enzymes on a solid material has a series of advantages over the use of free enzyme, including improved stability, enzyme separation, and reusability [4], and sometimes improving the substrate and support affinity [5]. There are several different methods that have been used for immobilization, for instance, entrapment ...
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Sulfidated nanoscale zero valent iron for in situ immobilization of hexavalent chromium in soil and response of indigenous microbes ... high reactivity, and easy separation (Ling and Zhang, 2017; Zou et ... This phenomenon can be explained by the fact that the attachment of sulfur to nZVI reduces the magnetic attraction and electrostatic ...
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In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe 3 O 4 nanoparticles (NPs). The Fe 3 O 4 NPs were synthesized by coprecipitation followed by modification with ammonium oleate. The surface-modified NPs were monodispersed and the particle size was about 13nm with 50.8emu/g …
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The immobilized cellulase was collected by magnetic separation and washed with sodium acetate buffer repeatedly. The residual enzyme in supernatant was detected to determine the enzyme loading on Me-. ... The in situ saccharification of microcrystalline cellulose catalyzed by Me-MBIE was selected to evaluate the effect of ratio of MBIE-to ...
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In situ magnetic separation (ISMS) constitutes an excellent adsorptive method for efficient extracellular protease removal during cultivation. In this work, the impact of semi-continuous ISMS on the overall protease yield has been investigated. Results reveal significant removal of the protease from Bacillus licheniformis cultivations ...
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Fe-biochar was separated from the treated soil using magnetic separation. In the cycling test, the biochar was separated using a magnet and regenerated by stirring in a 0.1 mol/L NaOH solution for 6 h and then dried. ... To demonstrate the enhanced As immobilization by magnetic biochar combined with microwave irradiation, a series of controlled ...
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Nano zero-valent iron (nZVI) has the advantages of small particle size, good in situ reactivity, magnetic separation and strong reducibility (Scott et al., 2011, Dickinson and Scott, 2010), and has been extensively investigated in the removal of heavy metal ions such as Pb 2+, AsO 4 3−, CrO 4 2− (Lv et al., 2012, Klas and Kirk, 2013, Qiu et ...
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Among them, magnetic adsorbents stand out as promising materials for heavy metal uptake, thanks to the benefits of rapid separation and recovery by an external magnetic field (Feng et al., 2020). Increasing attention has been paid to using magnetite (Fe 3 O 4 ) for sequestrating toxic metals because of its super magnetism, biocompatibility, and ...
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Request PDF | In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution | A new and simple method has been proposed ...
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Li YG, Gao HS, Li WL, Xing JM, Liu HZ (2009) In situ magnetic separation and immobilization of dibenzothiophene-desulfurizing bacteria. Bioresour Technol 100:5092–5096. Article CAS PubMed Google Scholar Liu PR, Zhang HL, Wang T, Yang WL, Hong Y, Hou YL (2016) Functional graphene-based magnetic nanocomposites as magnetic flocculant for ...
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This study provides a novel technique to achieve in-situ Se remediation and recovery by combining ZVI reduction and magnetic separation. Keywords: Environmental …
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In addition, the in situ formed Fe(III) oxide-hydroxide during Fe(VI) reduction exhibited a great potential to function as an effective sorbent/coagulant for hazardous As(III) and As(V) removal [27], [28]. Namely, the in situ interface oxidation-adsorption by Fe(VI)/PMS self-excitation could be achieved for PAA degradation and immobilization.
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